Johanna Urzua

Highly skilled molecular biologist with over 8 years of hands-on experience in laboratory research. Specializes in molecular biology techniques, microbiology, cell culture, and pathogen assays. Demonstrates a proven track record of contributing to scientific projects through expertise in DNA/RNA extraction, PCR, CRISPR gene editing, and protein analysis. Adept at handling both bacteria and fungi cultures under sterile conditions. Background in advanced microscopy and bioinformatics. Fluent in English, Spanish, and proficient in Swedish, showcasing a strong commitment to advancing scientific knowledge.

• Management of fungi and bacteria under sterile conditions
• Handling of adhesive cell cultures (Caco-2, MCF-7 and MDA-MB-231)
• Multimode plate reader-based assays such as ELISA, absorbance, fluorescence, and luminescence
• Flow cytometry and immunofluorescence analysis
• Broad experience with molecular biology technics, including DNA and RNA extraction, PCR, primer design, protein isolation, purification, enzymatic assays and western blotting
• Protein isolation and purification from E. coli using His-Trap columns.
• Cloning for mutagenesis, gene edition with CRISPR/Cas9 and sequencing
• Infection assays with pathogenic bacteria (Escherichia coli and Shigella flexneri)
• Management of stereo (Leica M205) and confocal (Leica LSM 780 and 800) microscope
• Bioinformatic and computing skills, including R language scripts, statistics, Excel, Word, and Prism 9

• Dr. Andrea Puhar, Senior research engineer, Department of Molecular Biology, Sweden, andrea.puhar@umu.se
• Dr. David Cisneros, Senior research engineer, Department of Molecular Biology, Sweden, d.cisneros@qub.ac.uk
• Dr. Judith Felten, Senior Lecturer, Department of Forest Genetics and Plant Physiology, Sweden, judith.lundberg-felten@geo.uu.se