KH NUR E SADID

• Greeted incoming customers, offered menu information, and documented orders
• Operated cash register to take payments and process orders
• Worked efficiently and precisely to fulfill customer needs and service goals.

In this thesis project, I conducted an inhibition study of the epsilon class glutathione S-transferase 8 (GSTE8) enzyme from the Anopheles gambiae mosquito. To carry out this project, GSTE8 was heterologously expressed in an E. coli system. The vector containing the GSTE8 gene was transformed into E. coli cells using heat shock transformation. The expressed protein was subsequently purified using IMAC affinity chromatography. The purification of the protein was confirmed by SDS-PAGE analysis. Finally, the purified protein was used for enzymological and inhibition studies.

• Conduct clinical and pathological analysis of blood and urine samples, including all biochemical parameters using Humalyzer/Bioanalyzer
• Perform bacterial and fungal culture, sample preservation, and staining techniques
• Extract DNA and RNA from bacterial and fungal samples for various applications
• Conduct RT‑PCR for SARS‑CoV‑2 detection
• Utilize ELISA to detect specific proteins and antibodies, including antibodies against SARS‑CoV‑2, Helicobacter pylori, and Mycobacterium tuberculosis
• Prepare detailed reports and documentation for regulatory submissions and internal audits
• Train newly employed staff in microbiological techniques, cleanroom protocols, and safety procedures.

In this project, I identified two pathogenic fungi responsible for banana leaf spot disease in the region of Rajshahi, Bangladesh. To identify the fungi, I first cultured them and isolated their DNA. The DNA was then amplified using fungal-specific primers, and the amplified DNA was sent for sequencing. By performing a BLAST search with the sequences, the pathogenic fungi were identified.