Dipti Singh

A dedicated postdoctoral researcher with over 8 years of experience in molecular biology and parasitology, specializing in Plasmodium biology and malaria pathogenesis. Expert in using genetic manipulation techniques, including CRISPR, to study Plasmodium gene function, protein localization, and their roles in host-parasite interactions. Proficient in experimental design, data analysis, and utilizing advanced technologies to uncover the molecular mechanisms of malaria. In addition, brings valuable experience in in vitro drug screening and assay development from a previous role in the pharmaceutical industry, where the optimization of high-throughput assays for drug discovery was a key focus.

• Cell & Molecular Biology: Expertise in mammalian cell culture, Plasmodium parasite culture, CRISPR, gene manipulation, molecular cloning, PCR, NGS, RNA isolation.
• Biochemical & Immunological Techniques: Recombinant protein expression, antibody production, ELISA, western blot, immunoprecipitation.
• Microscopy & Flow Cytometry: Light, fluorescence, confocal microscopy, GFP/mCherry sorting.
• Animal Handling: Handling and injection of rabbit and mouse models, including blood collection.
• Bioinformatics & Computer Skills: Sequence analysis (BLAST, ClustalW), primer designing, data analysis using Excel, Prism, EndNote, ImageJ, GENEDATA, and KNIME.

• Singh, D., et al., A Conserved Plasmodium Structural Integrity Maintenance Protein (SIMP) is associated with sporozoite membrane and is essential for maintaining shape and infectivity., Molecular Microbiology, 2022
• Lakshmeesha, K.N., Singh, D., et al., Biochemical and physiological investigations on adenosine 5’ monophosphate deaminase from Plasmodium spp., Molecular Microbiology, 2019
• Togiri, J., Singh, D., et al., Plasmodium berghei sporozoite specific genes- PbS10 and PbS23 are required for Exo-erythrocytic form development., Molecular & Biochemical Parasitology, 2019
• Singh, D., et al., Modulation of host cell SUMOylation facilitates efficient development of Plasmodium berghei and Toxoplasma gondii., Cellular Microbiology, 2017

• Dr. Kota Arun Kumar, Department of Animal Biology, University of Hyderabad, India, kaksl@uohyd.ac.in
• Dr. A Bindu Madav Reddy, Department of Animal Biology, University of Hyderabad, India, binduaramati@gmail.com
• Dr. Suresh Yenugu, Department of Animal Biology, University of Hyderabad, India, sureshsluohyd.ac.in